BioCyc Id	RXN-20202
Mass Balance Status	Balanced.
Direction Note	The reaction direction shown is in accordance with the direction in which it was curated.
EC Number	1.14.99.68 -- 4-aminobenzoate N-oxygenase
Enzyme Commission Synonyms	aurF (gene name)
Sub-reactions	4-aminobenzoate + reduced two electron carrier + dioxygen → 4-hydroxylaminobenzoate + oxidized electron carrier + H2O 4-hydroxylaminobenzoate + dioxygen → 4-nitrobenzoate + H2O

Enzyme Commission Summary

The enzyme, characterized from the bacterium Streptomyces thioluteus, catalyzes an early step in the biosynthesis of the antibiotic aureothin. It contains a carboxylate-bridged binuclear non-heme iron cluster. The native electron donor has not been identified, but is likely an iron-sulfur protein. The reaction mechanism involves formation of an extremely stable peroxo intermediate that catalyses three two-electron oxidations via a hydroxylamine and dihydroxylamine intermediates. cf EC 1.14.99.67, α-N-dichloroacetyl-p-aminophenylserinol N-oxygenase.

Citations: [He04, Lee06, Zocher07a, Choi08, Korboukh09, Li10]