Instances:
3.5.1.1 -- asparaginase

3.5.1.2 -- glutaminase

3.5.1.3 -- ω-amidase

3.5.1.4 -- amidase

3.5.1.5 -- urease

3.5.1.6 -- β-ureidopropionase

3.5.1.7 -- ureidosuccinase

3.5.1.8 -- formylaspartate deformylase

3.5.1.9 -- arylformamidase

3.5.1.10 -- formyltetrahydrofolate deformylase

3.5.1.11 -- penicillin amidase

3.5.1.12 -- biotinidase

3.5.1.13 -- aryl-acylamidase

3.5.1.14 -- N-acyl-aliphatic-L-amino acid amidohydrolase

3.5.1.15 -- aspartoacylase

3.5.1.16 -- acetylornithine deacetylase

3.5.1.17 -- acyl-lysine deacylase

3.5.1.18 -- succinyl-diaminopimelate desuccinylase

3.5.1.19 -- nicotinamidase

3.5.1.20 -- citrullinase

3.5.1.21 -- N-acetyl-β-alanine deacetylase

3.5.1.22 -- pantothenase

3.5.1.23 -- ceramidase

3.5.1.24 -- choloylglycine hydrolase

3.5.1.25 -- N-acetylglucosamine-6-phosphate deacetylase

3.5.1.26 -- N⁴-(β-N-acetylglucosaminyl)-L-asparaginase

3.5.1.28 -- N-acetylmuramoyl-L-alanine amidase

3.5.1.29 -- 2-(acetamidomethylene)succinate hydrolase

3.5.1.30 -- 5-aminopentanamidase

3.5.1.31 -- formylmethionine deformylase

3.5.1.32 -- hippurate hydrolase

3.5.1.33 -- N-acetylglucosamine deacetylase

3.5.1.35 -- D-glutaminase

3.5.1.36 -- N-methyl-2-oxoglutaramate hydrolase

3.5.1.38 -- glutamin-(asparagin-)ase

3.5.1.39 -- alkylamidase

3.5.1.40 -- acylagmatine amidase

3.5.1.41 -- chitin deacetylase

3.5.1.42 -- nicotinamide-nucleotide amidase

3.5.1.43 -- peptidyl-glutaminase

3.5.1.44 -- protein-glutamine glutaminase

3.5.1.46 -- 6-aminohexanoate-oligomer exohydrolase

3.5.1.47 -- N-acetyldiaminopimelate deacetylase

3.5.1.48 -- acetylspermidine deacetylase

3.5.1.49 -- formamidase

3.5.1.50 -- pentanamidase

3.5.1.51 -- 4-acetamidobutyryl-CoA deacetylase

3.5.1.52 -- peptide-N⁴-(N-acetyl-β-glucosaminyl)asparagine amidase

3.5.1.53 -- N-carbamoylputrescine amidase

3.5.1.54 -- allophanate hydrolase

3.5.1.55 -- long-chain-fatty-acyl-glutamate deacylase

3.5.1.56 -- N,N-dimethylformamidase

3.5.1.57 -- tryptophanamidase

3.5.1.58 -- N-benzyloxycarbonylglycine hydrolase

3.5.1.59 -- N-carbamoylsarcosine amidase

3.5.1.60 -- N-(long-chain-acyl)ethanolamine deacylase

3.5.1.62 -- acetylputrescine deacetylase

3.5.1.63 -- 4-acetamidobutyrate deacetylase

3.5.1.64 -- N^α-benzyloxycarbonylleucine hydrolase

3.5.1.65 -- theanine hydrolase

3.5.1.66 -- 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase

3.5.1.67 -- 4-methyleneglutaminase

3.5.1.68 -- N-formylglutamate deformylase

3.5.1.69 -- glycosphingolipid deacylase

3.5.1.70 -- aculeacin-A deacylase

3.5.1.71 -- N-feruloylglycine deacylase

3.5.1.72 -- D-benzoylarginine-4-nitroanilide amidase

3.5.1.73 -- carnitinamidase

3.5.1.74 -- chenodeoxycholoyltaurine hydrolase

3.5.1.75 -- urethanase

3.5.1.76 -- arylalkyl acylamidase

3.5.1.77 -- N-carbamoyl-D-amino-acid hydrolase

3.5.1.78 -- glutathionylspermidine amidase

3.5.1.79 -- phthalyl amidase

3.5.1.81 -- N-acyl-D-amino-acid deacylase

3.5.1.82 -- N-acyl-D-glutamate deacylase

3.5.1.83 -- N-acyl-D-aspartate deacylase

3.5.1.84 -- biuret amidohydrolase

3.5.1.85 -- (S)-N-acetyl-1-phenylethylamine hydrolase

3.5.1.86 -- mandelamide amidase

3.5.1.87 -- N-carbamoyl-L-amino-acid hydrolase

3.5.1.88 -- peptide deformylase

3.5.1.89 -- N-acetylglucosaminylphosphatidylinositol deacetylase

3.5.1.90 -- adenosylcobinamide hydrolase

3.5.1.91 -- N-substituted formamide deformylase

3.5.1.92 -- pantetheine hydrolase

3.5.1.93 -- glutaryl-7-aminocephalosporanic-acid acylase

3.5.1.94 -- γ-glutamyl-γ-aminobutyrate hydrolase

3.5.1.95 -- N-malonylurea hydrolase

3.5.1.96 -- succinylglutamate desuccinylase

3.5.1.97 -- acyl-homoserine-lactone acylase

3.5.1.98 -- histone deacetylase

3.5.1.99 -- fatty acid amide hydrolase

3.5.1.100 -- (R)-amidase

3.5.1.101 -- L-proline amide hydrolase

3.5.1.102 -- 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5′-monophosphate deformylase

3.5.1.103 -- N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-α-D-glucopyranoside deacetylase

3.5.1.104 -- peptidoglycan-N-acetylglucosamine deacetylase

3.5.1.105 -- chitin disaccharide deacetylase

3.5.1.106 -- N-formylmaleamate deformylase

3.5.1.107 -- maleamate amidohydrolase

3.5.1.108 -- UDP-3-O-acyl-N-acetylglucosamine deacetylase

3.5.1.109 -- sphingomyelin deacylase

3.5.1.110 -- ureidoacrylate amidohydrolase

3.5.1.111 -- 2-oxoglutaramate amidase

3.5.1.112 -- 2′-N-acetylparomamine deacetylase

3.5.1.113 -- 2′′′-acetyl-6′′′-hydroxyneomycin C deacetylase

3.5.1.114 -- N-acyl-aromatic-L-amino acid amidohydrolase

3.5.1.115 -- mycothiol S-conjugate amidase

3.5.1.116 -- ureidoglycolate amidohydrolase

3.5.1.117 -- 6-aminohexanoate-oligomer endohydrolase

3.5.1.118 -- γ-glutamyl hercynylcysteine S-oxide hydrolase

3.5.1.119 -- Pup amidohydrolase

3.5.1.121 -- protein N-terminal asparagine amidohydrolase

3.5.1.122 -- protein N-terminal glutamine amidohydrolase

3.5.1.123 -- γ-glutamylanilide hydrolase

3.5.1.124 -- protein deglycase

3.5.1.125 -- N²-acetyl-L-2,4-diaminobutanoate deacetylase

3.5.1.126 -- oxamate amidohydrolase

3.5.1.127 -- jasmonoyl-L-amino acid hydrolase

3.5.1.128 -- deaminated glutathione amidase

3.5.1.129 -- N⁵-(cytidine 5′-diphosphoramidyl)-L-glutamine hydrolase

3.5.1.130 -- [amino group carrier protein]-lysine hydrolase

3.5.1.131 -- 1-carboxybiuret hydrolase

3.5.1.132 -- [amino group carrier protein]-ornithine hydrolase

3.5.1.133 -- N^α-acyl-L-glutamine aminoacylase

3.5.1.134 -- (indol-3-yl)acetyl-L-aspartate hydrolase

3.5.1.135 -- N⁴-acetylcytidine amidohydrolase

3.5.1.136 -- N,N′-diacetylchitobiose non-reducing end deacetylase

3.5.1.137 -- N-methylcarbamate hydrolase

3.5.1.138 -- lipoamidase

3.5.1.M4 -- N-acetyl-D-glucosaminyl-N-acetyl-tunicaminyl-uracil N-deacetylase

3.5.1.M7 -- indole-3-acetyl-L-amino acid hydrolase

3.5.1.M13 -- lanatoside 15'-O-acetylesterase

3.5.1.M15 -- (S)-malyl N-acetyl-α-D-glucosaminide deacetylase

3.5.1.M19 -- linuron hydrolase

3.5.1.M20 -- N-methoxy-N-methyl phenylurea hydrolase

3.5.1.M21 -- phenylurea hydrolase

3.5.1.M23 -- N-acetyl-L-glutamate deacetylase

Unofficial Reactions:
N-acetyl-L-cysteine + H₂O → L-cysteine + acetate,
2-formylaminobenzaldehyde + H₂O → O-aminobenzaldehyde + formate + H⁺,
ochratoxin A + H₂O → ochratoxin α + L-phenylalanine,
poly-β-1,6-N-acetyl-D-glucosamine + H₂O → a partially deacetylated poly-β-1,6-N-acetyl-D-glucosamine + acetate,
N-(4-aminobenzoyl)-L-glutamate + H₂O → 4-aminobenzoate + L-glutamate,
N-(β-D-glucosyl)-(indol-3-yl)acetyl-L-glutamate + H₂O → N-β-D-glucosyl-(indol-3-yl)acetate + L-glutamate,
N-acetyl-β-D-glucosaminyl-(1→4)-D-glucosamine + H₂O → chitobiose + acetate,
(R)-4'-phosphopantothenoyl-L-cysteine + H₂O + H⁺ → cysteamine + (R)-4'-phosphopantothenate + CO₂,
2'''-acetyl-6'''-hydroxyparomomycin + H₂O → 6'''-hydroxyparomomycin + acetate,
a [protein]-N⁶-acetyl-L-lysine + H₂O → a [protein]-L-lysine + acetate,
formylaminopyrimidine + H₂O → aminomethylpyrimidine + formate,
N-acetyl-6-diazo-5-oxo-L-norleucyl-[6-diazo-5-oxo-L-norleucine-carrier protein] + H₂O → 6-diazo-5-oxo-L-norleucyl-[6-diazo-5-oxo-L-norleucine-carrier protein] + acetate,
N⁵-phospho-L-glutamine + H₂O → phosphoramidate + L-glutamate + H⁺,
5-acetamidovalerate + H₂O → 5-aminopentanoate + acetate,
3-methylglutaconyl-CoA + N⁵-hydroxy-L-ornithine → methylglutaconyl hydroxy ornithine + coenzyme A,
N,N',N''-triacetylchitotriose + H₂O → N,N''-diacetylchitotriose + acetate,
2-chloro-N-(2,6-diethylphenyl)acetamide + H₂O → 2,6-diethylaniline + 2-chloroacetate + H⁺,
acetaminophen + H₂O → 4-aminophenol + acetate + H⁺,
4-hydroxy-2-nonenal-N-acetyl-L-cysteine + H₂O → S-(4-hydroxy-nonenal-3-yl)-L-cysteine + acetate,
echinocandin B + H₂O → echinocandin B nucleus + linoleate,
(Z)-N,N'-(but-2-ene-2,3-diyl)diacetamide + H₂O → N-(3-oxobutan-2-yl)acetamide + acetamide,
N-(3-hydroxybutan-2-yl)acetamide + H₂O → 3-amino-2-butanol + acetate,
4-formamido-2-oxobut-3-enoate + H₂O → 4-amino-2-oxobut-3-enoate + formate,
5-ureidoimidazole-4-carboxylate + H₂O + 2 H⁺ → 5-aminoimidazole-4-carboxylate + CO₂ + ammonium,
N,N'-diacetylchitobiose 6'-phosphate + H₂O → 6-phospho-β-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate,
N-formyldemecolcine + H₂O → demecolcine + formate,
UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronate + H₂O → UDP-3-acetamido-2-amino-2,3-dideoxy-α-D-glucuronate + acetate,
myristoylated ferribactin + H₂O → ferribactin + tetradecanoate,
N-(1-deoxy-6-phospho-D-fructos-1-yl)-L-aspartate + H₂O → D-glucose 6-phosphate + L-aspartate,
N-β-D-glucosyl-(indol-3-yl)acetyl-L-aspartate + H₂O → N-β-D-glucosyl-(indol-3-yl)acetate + L-aspartate

Enzymes and Genes:
N-(1-deoxy-6-phospho-D-fructos-1-yl)-L-aspartate deglycase: fraB ( Salmonella enterica enterica serovar Typhimurium str. LT2 )
myristoylated ferribactin deacylase: pvdQ ( Pseudomonas aeruginosa PAO1 )
UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronate deacetylase: vimE ( Porphyromonas gingivalis )
N-formyldemecolcine deformylase [multifunctional] ( Colchicum autumnale )
chitooligosaccharide monodeacetylase ChbG: chbG ( Escherichia coli K-12 substr. MG1655 )
4-ureido-5-imidazolecarboxylate amidohydrolase ( Clostridium cylindrosporum )
4-formamido-2-oxobut-3-enoate deformylase: kpiB ( Arthrobacter sp. IN13 )
4-formamido-2-oxobut-3-enoate deformylase: pydB ( Rhizobium sp. TAL1145 )
(Z)-N,N'-(but-2-ene-2,3-diyl)diacetamide hydrolase: tpdC ( Rhodococcus sp. TMP1 )
echinocandin B deacylase ( Actinoplanes utahensis )
aminoacylase 3: ACY3 ( Homo sapiens )
chitin oligosaccharide deacetylase: cod ( Vibrio cholerae O1 biovar El Tor str. N16961 )
N-hydroxyornithine acylase: Fer5 ( Mycosarcoma maydis )
N-acetyl-6-diazo-5-oxo-L-norleucyl-[6-diazo-5-oxo-L-norleucine-carrier protein] deacetylase: azpG ( Peterkaempfera griseoplana )
N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase: ylmB ( Halalkalibacterium halodurans )
2'''-acetyl-6'''-hydroxyparomomycin deacetylase: livD ( Streptomyces lividus )
p-aminobenzoyl-glutamate hydrolase: abgA, abgB ( Escherichia coli K-12 substr. MG1655 )
p-aminobenzoylglutamate hydrolase ( Arabidopsis thaliana )
poly-β-1,6-N-acetyl-D-glucosamine N-deacetylase and β-1,6 glycoside hydrolase: pgaB ( Escherichia coli K-12 substr. MG1655 )
N-acyl-L-amino acid amidohydrolase: AbgB ( Alcaligenes faecalis )
N-acetyl-L-cysteine deacetylase: sndA ( Bacillus subtilis subtilis 168 )
N-acetyl-sulfur-metabolite deacetylase: sndB ( Bacillus subtilis subtilis 168 )
N-acetyl amino acid acetylase: sndC ( Bacillus subtilis subtilis 168 )