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FBA Tutorial
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Pathway Tools
FBA Tutorial
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MetaCyc Enzyme: long-chain fatty acid ω-hydroxylase

Gene: CYP704B1 Accession Number: AT1G69500 (MetaCyc)

Species: Arabidopsis thaliana col

Unification Links: ArrayExpress:Q8GWJ5 , Pride:Q8GWJ5 , Protein Model Portal:Q8GWJ5 , String:3702.AT1G69500.1-P , UniProt:Q8GWJ5

Relationship Links: InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463

Gene-Reaction Schematic: ?

Credits:
Created 24-Feb-2011 by Zhang P , TAIR


Enzymatic reaction of: long-chain fatty acid ω-hydroxylase

a fatty acid + 2 NADPH + oxygen + H+ <=> a ω-hydroxy fatty acid + 2 NADP+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: sporopollenin precursor biosynthesis , alkane oxidation

Summary:
The cDNA of CYP704B1 was expressed in yeast cells. The enzyme activity of CYP704B1 was demonstrated in an in vitro enzyme assay, where palmitate was transformed to 16-hydroxypalmitate. In addition to palmitate, CYP704B1 also acted on oleate, linoleate, linolenate and 9,10-epoxystearate. Laurate was a poor substrate. Phenolics such as cinnamate, p-coumarate, ferulate, sinapate and p-coumaroyl shikimate were not metabolized by CYP704B1.

CYP704B1 had an anther-specific expression pattern and is highly coexpressed with CYP703A2, an in-chain fatty acid hydroxylase involved in exine biosynthesis. CYP704B1 mutant plants had impaired pollen walls that lack a normal exine layer.


References

Dobritsa09: Dobritsa AA, Shrestha J, Morant M, Pinot F, Matsuno M, Swanson R, Moller BL, Preuss D (2009). "CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis." Plant Physiol 151(2);574-89. PMID: 19700560


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 38:D473-D479 2010
Page generated by SRI International Pathway Tools version 18.0 on Sun Sep 21, 2014, biocyc13.