Parent Class:

EC-Numbers → 6 -- Ligases → 6.4 -- Forming carbon-carbon bonds → 6.4.1 -- Ligases that form carbon-carbon bonds

Synonyms: oxalosuccinate synthetase, carboxylating factor for ICDH (incorrect), CFI, OGC

Unification Links: BRENDA:6.4.1.7, ENZYME:6.4.1.7, IUBMB-ExplorEnz:6.4.1.7

Reaction:
ATP + 2-oxoglutarate + CO₂ + H₂O → oxalosuccinate + ADP + phosphate + 2 H⁺

Enzymes and Genes:
2-oxoglutarate carboxylase: cfiB, cfiA ( Hydrogenobacter thermophilus )

Summary:
A biotin-containing enzyme that requires Mg²⁺ for activity. It was originally thought [Aoshima04] that this enzyme was a promoting factor for the carboxylation of 2-oxoglutarate by EC 1.1.1.41, isocitrate dehydrogenase (NAD+), but this has since been disproved [Aoshima06]. The product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 [Aoshima06].

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References

Aoshima04: Aoshima M, Ishii M, Igarashi Y (2004). "A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6." Mol Microbiol 51(3);791-8. PMID: 14731279

Aoshima06: Aoshima M, Igarashi Y (2006). "A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6." Mol Microbiol 62(3);748-59. PMID: 17076668

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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