Parent Class:

EC-Numbers → 3 -- Hydrolases → 3.5 -- Acting on carbon-nitrogen bonds, other than peptide bonds → 3.5.1 -- In linear amides

Synonyms: HP310, PgdA, SpPgdA, BC1960, peptidoglycan deacetylase, N-acetylglucosamine deacetylase, peptidoglycan GlcNAc deacetylase, peptidoglycan N-acetylglucosamine deacetylase, PG N-deacetylase

Systematic Name: peptidoglycan-N-acetylglucosamine amidohydrolase

Unification Links: BRENDA:3.5.1.104, ENZYME:3.5.1.104, IUBMB-ExplorEnz:3.5.1.104

Reaction:
a peptidoglycan + H₂O → a deacetylated peptidoglycan + acetate

Enzymes and Genes:
peptidoglycan-N-acetylglucosamine deacetylase: BC_1960 ( Bacillus cereus )

Summary:
Modification of peptidoglycan by N-deacetylation is an important factor in virulence of Helicobacter pylori, Listeria monocytogenes and Streptococcus suis [Wang09, Popowska09, Fittipaldi08]. The enzyme from Streptococcus pneumoniae is a metalloenzyme using a His-His-Asp zinc-binding triad with a nearby aspartic acid and histidine acting as the catalytic base and acid, respectively [Blair05].

Citations: [Psylinakis05, Tsalafouta08]

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References

Blair05: Blair DE, Schuttelkopf AW, MacRae JI, van Aalten DM (2005). "Structure and metal-dependent mechanism of peptidoglycan deacetylase, a streptococcal virulence factor." Proc Natl Acad Sci U S A 102(43);15429-34. PMID: 16221761

Fittipaldi08: Fittipaldi N, Sekizaki T, Takamatsu D, Dominguez-Punaro Mde L, Harel J, Bui NK, Vollmer W, Gottschalk M (2008). "Significant contribution of the pgdA gene to the virulence of Streptococcus suis." Mol Microbiol 70(5);1120-35. PMID: 18990186

Popowska09: Popowska M, Kusio M, Szymanska P, Markiewicz Z (2009). "Inactivation of the wall-associated de-N-acetylase (PgdA) of Listeria monocytogenes results in greater susceptibility of the cells to induced autolysis." J Microbiol Biotechnol 19(9);932-45. PMID: 19809250

Psylinakis05: Psylinakis E, Boneca IG, Mavromatis K, Deli A, Hayhurst E, Foster SJ, Varum KM, Bouriotis V (2005). "Peptidoglycan N-acetylglucosamine deacetylases from Bacillus cereus, highly conserved proteins in Bacillus anthracis." J Biol Chem 280(35);30856-63. PMID: 15961396

Tsalafouta08: Tsalafouta A, Psylinakis E, Kapetaniou EG, Kotsifaki D, Deli A, Roidis A, Bouriotis V, Kokkinidis M (2008). "Purification, crystallization and preliminary X-ray analysis of the peptidoglycan N-acetylglucosamine deacetylase BC1960 from Bacillus cereus in the presence of its substrate (GlcNAc)6." Acta Crystallogr Sect F Struct Biol Cryst Commun 64(Pt 3);203-5. PMID: 18323609

Wang09: Wang G, Olczak A, Forsberg LS, Maier RJ (2009). "Oxidative stress-induced peptidoglycan deacetylase in Helicobacter pylori." J Biol Chem 284(11);6790-800. PMID: 19147492

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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