Parent Class:

EC-Numbers → 3 -- Hydrolases → 3.4 -- Acting on peptide bonds (peptide hydrolases) → 3.4.22 -- Cysteine endopeptidases

Synonyms: allergen Der f 1, allergen Der p 1, antigen Der p 1, antigen Eur m 1, antigen Pso o 1, major mite fecal allergen Der p 1, Der p 1, Der f 1, Eur m 1, endopeptidase 1 (mite)

Unification Links: BRENDA:3.4.22.65, ENZYME:3.4.22.65, IUBMB-ExplorEnz:3.4.22.65

Reaction:
peptidase 1 substrate + H₂O → 2 peptide + 2 H⁺

Summary:
This enzyme, derived from the house dust mite, is a major component of the allergic immune response. The substrate specificity of this enzyme is not altogether clear; it cleaves the low-affinity IgE receptor CD23 at 298-Glu-|-Ser-299 and 155-Ser-|-Ser-156. It also cleaves the pulmonary structural proteins occludin and claudin at Leu-|-Leu, Asp-|-Leu and at Gly-|-Thr bonds, and it can also cleave the alpha subunit of the interleukin-2 (IL-2) receptor (CD25). Using a positional scanning combinatorial library, it was found that the major substrate-specificity determinant is for Ala in the P2 position. The enzyme shows only a slight preference for basic amino acids in the P1 and P3 positions and a preference for aliphatic amino acids such as Ile, Pro, Val, Leu and norleucine in the P4 position. Belongs to peptidase family C1A.

Citations: [Kalsheker96, Schulz98, Schulz98a, Harris04, Takai05]

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References

Harris04: Harris J, Mason DE, Li J, Burdick KW, Backes BJ, Chen T, Shipway A, Van Heeke G, Gough L, Ghaemmaghami A, Shakib F, Debaene F, Winssinger N (2004). "Activity profile of dust mite allergen extract using substrate libraries and functional proteomic microarrays." Chem Biol 11(10);1361-72. PMID: 15489163

Kalsheker96: Kalsheker NA, Deam S, Chambers L, Sreedharan S, Brocklehurst K, Lomas DA (1996). "The house dust mite allergen Der p1 catalytically inactivates alpha 1-antitrypsin by specific reactive centre loop cleavage: a mechanism that promotes airway inflammation and asthma." Biochem Biophys Res Commun 221(1);59-61. PMID: 8660343

Schulz98: Schulz O, Sewell HF, Shakib F (1998). "A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermatophagoides pteronyssinus." Mol Pathol 51(4);222-4. PMID: 9893750

Schulz98a: Schulz O, Sewell HF, Shakib F (1998). "Proteolytic cleavage of CD25, the alpha subunit of the human T cell interleukin 2 receptor, by Der p 1, a major mite allergen with cysteine protease activity." J Exp Med 187(2);271-5. PMID: 9432986

Takai05: Takai T, Kato T, Sakata Y, Yasueda H, Izuhara K, Okumura K, Ogawa H (2005). "Recombinant Der p 1 and Der f 1 exhibit cysteine protease activity but no serine protease activity." Biochem Biophys Res Commun 328(4);944-52. PMID: 15707969

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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