Parent Class:

EC-Numbers → 3 -- Hydrolases → 3.4 -- Acting on peptide bonds (peptide hydrolases) → 3.4.22 -- Cysteine endopeptidases

Synonyms: Arg-gingipain, gingipain-1, argingipain, Arg-gingivain-55 proteinase, Arg-gingivain-70 proteinase, Arg-gingivain-75 proteinase, arginine-specific cysteine protease, arginine-specific gingipain, arginine-specific gingivain, RGP-1, RGP

Unification Links: BRENDA:3.4.22.37, ENZYME:3.4.22.37, IUBMB-ExplorEnz:3.4.22.37

Reaction:
protein + H₂O → 2 peptide + 2 H⁺

Enzymes and Genes:
Arg-gingipain B: rgpB ( Porphyromonas gingivalis W50 )
Arg-gingipain A: rgpA ( Porphyromonas gingivalis W50 )

Summary:
This enzyme catalyzes hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1. A secreted endopeptidase from the bacterium Porphyromonas gingivalis. Strongly activated by glycine [Chen92], and stabilized by Ca²⁺. Precursor molecule contains a hemagglutinin domain [Kirszbaum95], [Pavloff95] Misleadingly described in some literature as "trypsin-like", being a cysteine peptidase, type example of family C25.

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References

Chen92: Chen Z, Potempa J, Polanowski A, Wikstrom M, Travis J (1992). "Purification and characterization of a 50-kDa cysteine proteinase (gingipain) from Porphyromonas gingivalis." J Biol Chem 267(26);18896-901. PMID: 1527017

Kirszbaum95: Kirszbaum L, Sotiropoulos C, Jackson C, Cleal S, Slakeski N, Reynolds EC (1995). "Complete nucleotide sequence of a gene prtR of Porphyromonas gingivalis W50 encoding a 132 kDa protein that contains an arginine-specific thiol endopeptidase domain and a haemagglutinin domain." Biochem Biophys Res Commun 207(1);424-31. PMID: 7857299

Pavloff95: Pavloff N, Potempa J, Pike RN, Prochazka V, Kiefer MC, Travis J, Barr PJ (1995). "Molecular cloning and structural characterization of the Arg-gingipain proteinase of Porphyromonas gingivalis. Biosynthesis as a proteinase-adhesin polyprotein." J Biol Chem 270(3);1007-10. PMID: 7836351

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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