Parent Class:

EC-Numbers → 3 -- Hydrolases → 3.4 -- Acting on peptide bonds (peptide hydrolases) → 3.4.21 -- Serine endopeptidases

Synonyms: Streptomyces griseus protease B, pronase B, serine proteinase B, Streptomyces griseus proteinase B, Streptomyces griseus proteinase 1, Streptomyces griseus serine proteinase B

Unification Links: BRENDA:3.4.21.81, ENZYME:3.4.21.81, IUBMB-ExplorEnz:3.4.21.81

Reaction:
protein + H₂O → 2 peptide + 2 H⁺

Summary:
This enzyme catalyzes hydrolysis of proteins with trypsin-like specificity. From Streptomyces griseus. A component of Pronase, in peptidase family S1 (trypsin family), distinct from Streptomyces trypsin

Citations: [Jurasek69, Fujinaga82, Read83, Henderson87, Greenblatt89]

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References

Fujinaga82: Fujinaga M, Read RJ, Sielecki A, Ardelt W, Laskowski M, James MN (1982). "Refined crystal structure of the molecular complex of Streptomyces griseus protease B, a serine protease, with the third domain of the ovomucoid inhibitor from turkey." Proc Natl Acad Sci U S A 79(16);4868-72. PMID: 6750612

Greenblatt89: Greenblatt HM, Ryan CA, James MN (1989). "Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1 A resolution." J Mol Biol 205(1);201-28. PMID: 2494344

Henderson87: Henderson G, Krygsman P, Liu CJ, Davey CC, Malek LT (1987). "Characterization and structure of genes for proteases A and B from Streptomyces griseus." J Bacteriol 169(8);3778-84. PMID: 3112129

Jurasek69: Jurasek L, Fackre D, Smillie LB (1969). "Remarkable homology about the disulfide bridges of a trypsin-like enzyme from Streptomyces griseus." Biochem Biophys Res Commun 37(1);99-105. PMID: 4899581

Read83: Read RJ, Fujinaga M, Sielecki AR, James MN (1983). "Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8-A resolution." Biochemistry 22(19);4420-33. PMID: 6414511

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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