Parent Class:

EC-Numbers → 3 -- Hydrolases → 3.1 -- Acting on ester bonds → 3.1.4 -- Phosphoric diester hydrolases

Synonyms: 5′-exonuclease, 5′-phosphodiesterase, 5′-nucleotide phosphodiesterase, oligonucleate 5′-nucleotidohydrolase, 5′ nucleotide phosphodiesterase/alkaline phosphodiesterase I, 5′-NPDase, 5′-PDase, 5′-PDE, 5’NPDE, alkaline phosphodiesterase, nucleotide pyrophosphatase/phosphodiesterase I, orthophosphoric diester phosphohydrolase, PDE I, phosphodiesterase (ambiguous), exonuclease I

Systematic Name: oligonucleotide 5′-nucleotidohydrolase

Unification Links: BRENDA:3.1.4.1, ENZYME:3.1.4.1, IUBMB-ExplorEnz:3.1.4.1

Reaction:
a double-stranded oligonucleotide + H₂O → a double-stranded oligonucleotide + a nucleoside 5'-monophosphate

Summary:
This enzyme hydrolytically removes 5-nucleotides successively from the 3-hydroxy termini of 3-hydroxy-terminated oligonucleotides.

Hydrolyses both ribonucleotides and deoxyribonucleotides. Has low activity towards polynucleotides. A 3-phosphate terminus on the substrate inhibits hydrolysis.

Citations: [Khorana61]

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References

Khorana61: Khorana, G.H. (1961). "Phosphodiesterases." In: Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 5, Academic Press, New York,.

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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