Pathway Tools Tip

Green "?" Buttons Provide Help

Click on the small help buttons containing a "?" within a green circle for explanations of nearby elements of a page

To unsubscribe from tips, login or create an account.

Synonyms: vitamine B₆ (pyridoxine) phosphatase, PLP phosphatase, vitamin B₆-phosphate phosphatase, PNP phosphatase

Systematic Name: pyridoxal-5′-phosphate phosphohydrolase

Unification Links: BRENDA:3.1.3.74, ENZYME:3.1.3.74, IUBMB-ExplorEnz:3.1.3.74

Reaction:
pyridoxal 5'-phosphate + H₂O → pyridoxal + phosphate

Unofficial Reactions:
pyridoxamine 5'-phosphate + H₂O → pyridoxamine + phosphate,
pyridoxine 5'-phosphate + H₂O → pyridoxine + phosphate

Enzymes and Genes:
pyridoxal phosphate/fructose-1,6-bisphosphate phosphatase: ybhA ( Escherichia coli K-12 substr. MG1655 )
phosphosugar phosphatase YigL: yigL ( Escherichia coli K-12 substr. MG1655 )
HMP-PP phosphatase: cof ( Escherichia coli K-12 substr. MG1655 )
pyridoxal phosphate phosphatase: PHOSPHO2 ( Homo sapiens )
alkaline phosphatase: phoA ( Escherichia coli K-12 substr. MG1655 )

Summary:
This enzyme is specific for phosphorylated vitamin B6 compounds: it acts not only on pyridoxal 5'-phosphate (PLP), but also on pyridoxine 5'-phosphate (PNP), pyridoxamine 5'-phosphate (PMP), 4-pyridoxate 5'-phosphate and 4-deoxypyridoxine 5'-phosphate. This reaction can also be carried out by EC 3.1.3.1, alkaline phosphatase and EC 3.1.3.2, acid phosphatase, but these enzymes have very broad substrate specificities.

Citations: [Fonda92, Fonda95, Jang03]

References

Fonda92: Fonda ML (1992). "Purification and characterization of vitamin B6-phosphate phosphatase from human erythrocytes." J Biol Chem 267(22);15978-83. PMID: 1322411

Fonda95: Fonda ML, Zhang YN (1995). "Kinetic mechanism and divalent metal activation of human erythrocyte pyridoxal phosphatase." Arch Biochem Biophys 320(2);345-52. PMID: 7625842

Jang03: Jang YM, Kim DW, Kang TC, Won MH, Baek NI, Moon BJ, Choi SY, Kwon OS (2003). "Human pyridoxal phosphatase. Molecular cloning, functional expression, and tissue distribution." J Biol Chem 278(50);50040-6. PMID: 14522954