Parent Class:

EC-Numbers → 3 -- Hydrolases → 3.1 -- Acting on ester bonds → 3.1.11 -- Exodeoxyribonucleases producing 5'-phosphomonoesters

Synonyms: lambda exonuclease, phage lambda-induced exonuclease, Escherichia coli exonuclease IV, E. coli exonuclease IV, exodeoxyribonuclease IV, exonuclease IV

Unification Links: BRENDA:3.1.11.3, ENZYME:3.1.11.3, IUBMB-ExplorEnz:3.1.11.3

Supersedes EC number: 3.1.4.28

Reaction:
double stranded DNA + H₂O → double stranded DNA + a nucleoside 5'-monophosphate

Summary:
This enzyme catalyzes exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates.

Preference for double-stranded DNA. Does not attack single-strand breaks.

Citations: [Little67, Lindahl69]

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References

Lindahl69: Lindahl T, Gally JA, Edelman GM (1969). "Deoxyribonuclease IV: a new exonuclease from mammalian tissues." Proc Natl Acad Sci U S A 62(2);597-603. PMID: 5256235

Little67: Little JW (1967). "An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction." J Biol Chem 242(4);679-86. PMID: 6017737

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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