Parent Class:

EC-Numbers → 2 -- Transferases → 2.1 -- Transferring one-carbon groups → 2.1.1 -- Methyltransferases

Synonyms: NMT1 (gene name), METTL11A (gene name)

Systematic Name: S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-[protein] methyltransferase

Unification Links: BRENDA:2.1.1.244, ENZYME:2.1.1.244, IUBMB-ExplorEnz:2.1.1.244

Reaction:
2 S-adenosyl-L-methionine + an N-terminal-PPK-[protein] → 2 S-adenosyl-L-homocysteine + an N terminal N,N-dimethyl-PPK-[protein] + 2 H⁺,
3 S-adenosyl-L-methionine + an N-terminal-(A/S)PK-[protein] → 3 S-adenosyl-L-homocysteine + an N terminal N,N,N-trimethyl-(A/S)PK-[protein] + 3 H⁺

Enzymes and Genes:
protein N-terminal methyltransferase: NTMT1 ( Homo sapiens )

Summary:
The enzyme specifically catalyses mono- or di-methylation (Ala, Ser or Pro) or tri-methylation (Ala or Ser) of the exposed α-amino group in the [Ala/Pro/Ser]-Pro-Lys motif. The protein from Saccharomyces cerevisiae shows the highest activity towards the Pro-Pro-Lys-motif [Webb10]. The Pro-Lys in positions 2-3 cannot be exchanged for other amino acids [Webb10, Tooley10].

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References

Tooley10: Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG (2010). "NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein." Nature 466(7310);1125-8. PMID: 20668449

Webb10: Webb KJ, Lipson RS, Al-Hadid Q, Whitelegge JP, Clarke SG (2010). "Identification of protein N-terminal methyltransferases in yeast and humans." Biochemistry 49(25);5225-35. PMID: 20481588

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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