Parent Class:

EC-Numbers → 1 -- Oxidoreductases → 1.14 -- Acting on paired donors, with incorporation or reduction of molecular oxygen → 1.14.12 -- With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Synonyms: biphenyl dioxygenase

Systematic Name: biphenyl,NADH:oxygen oxidoreductase (2,3-hydroxylating)

Unification Links: BRENDA:1.14.12.18, ENZYME:1.14.12.18, IUBMB-ExplorEnz:1.14.12.18

Reaction:
biphenyl + NADH + dioxygen + H⁺ → cis-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD⁺

Unofficial Reactions:
4-bromodiphenyl ether + NADH + dioxygen + H⁺ = 4-bromo-2,3-dihydrodiol-diphenyl ether + NAD⁺,
diphenyl ether + NADPH + dioxygen + H⁺ → (1S,2S)-3-phenoxycyclohexa-3,5-diene-1,2-diol + NADP⁺

Enzymes and Genes:
biphenyl 2,3-dioxygenase: bphAb, bphAa, bphAd, bphAc ( Rhodococcus jostii RHA1 )
ethylbenzene dioxygenase: etbAb1, etbAa1 ( Rhodococcus jostii RHA1 )
ethylbenzene dioxygenase: etbAb2, etbAa2, etbAc ( Rhodococcus jostii RHA1 )
chlorobenzene dioxygenase: tecA4, tecA3, tecA2, tecA1 ( Cupriavidus sp. PS12 )
biphenyl 2,3-dioxygenase: bphA1, bphA2, bphA3, bphA4 ( Cupriavidus sp. WS )

Summary:
The enzyme from Paraburkholderia xenovorans LB400 is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske-type iron-sulfur cluster and a catalytic mononuclear nonheme iron center.

Citations: [Haddock, Haddock95, Broadus98]

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References

Broadus98: Broadus RM, Haddock JD (1998). "Purification and characterization of the NADH:ferredoxinBPH oxidoreductase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400." Arch Microbiol 170(2);106-12. PMID: 9683647

Haddock: Haddock JD, Pelletier DA, Gibson DT "Purification and properties of ferredoxinBPH, a component of biphenyl 2,3-dioxygenase of Pseudomonas sp strain LB400." J Ind Microbiol Biotechnol 19(5-6);355-9. PMID: 9451832

Haddock95: Haddock JD, Gibson DT (1995). "Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400." J Bacteriol 177(20);5834-9. PMID: 7592331

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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