Parent Class:

EC-Numbers → 1 -- Oxidoreductases → 1.13 -- Acting on single donors with incorporation of molecular oxygen → 1.13.11 -- With incorporation of two atoms of oxygen

Synonyms: latex clearing protein, lcp (gene name), roxB (gene name)

Systematic Name: cis-1,4-polyisoprene:oxygen dioxygenase (endo-cleaving)

Unification Links: BRENDA:1.13.11.87, ENZYME:1.13.11.87, IUBMB-ExplorEnz:1.13.11.87

Reaction:
cis-1,4-polyisoprene + n dioxygen → j (4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal + k (4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal + l (4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal + m (4Z,8Z,12Z)-4,8,12-trimethyl-16-oxoheptadeca-4,8,12-trienal

Enzymes and Genes:
endo-cleaving rubber dioxygenase RoxB: roxB ( Xanthomonas sp. 35Y )
endo-cleaving rubber dioxygenase Lcp: lcp ( Streptomyces sp. K30 )

Summary:
The enzyme catalyzes the cleavage of the double bonds in natural and synthetic rubber, producing a mixture of C20, C25, C30, and higher oligo-isoprenoids with ketone and aldehyde groups at their ends. Two unrelated enzymes are known to possess this activity - the enzyme from Streptomyces sp. K30 (Lcp) contains a b-type cytochrome, while the enzyme from Xanthomonas sp. 35Y (RoxB) contains two c-type cytochromes. Both enzymes attack the substrate at random locations, and are not able to cleave the C35 or smaller products into shorter fragments.

Citations: [Rose05, Yikmis08, Birke14, Rother16, Ilcu17, Birke17]

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References

Birke14: Birke J, Jendrossek D (2014). "Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms." Appl Environ Microbiol 80(16);5012-20. PMID: 24907333

Birke17: Birke J, Rother W, Jendrossek D (2017). "RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp)." Appl Environ Microbiol 83(14). PMID: 28500046

Ilcu17: Ilcu L, Rother W, Birke J, Brausemann A, Einsle O, Jendrossek D (2017). "Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber." Sci Rep 7(1);6179. PMID: 28733658

Rose05: Rose K, Tenberge KB, Steinbuchel A (2005). "Identification and characterization of genes from Streptomyces sp. strain K30 responsible for clear zone formation on natural rubber latex and poly(cis-1,4-isoprene) rubber degradation." Biomacromolecules 6(1);180-8. PMID: 15638519

Rother16: Rother W, Austen S, Birke J, Jendrossek D (2016). "Cleavage of Rubber by the Latex Clearing Protein (Lcp) of Streptomyces sp. Strain K30: Molecular Insights." Appl Environ Microbiol 82(22);6593-6602. PMID: 27590810

Yikmis08: Yikmis M, Arenskotter M, Rose K, Lange N, Wernsmann H, Wiefel L, Steinbuchel A (2008). "Secretion and transcriptional regulation of the latex-clearing protein, Lcp, by the rubber-degrading bacterium Streptomyces sp. strain K30." Appl Environ Microbiol 74(17);5373-82. PMID: 18606806

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 46(D1):D633-D639 2018
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